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Cells of Bacillus megaterium immobilized due to incorporation into polyacrylamide gel retained their viability. They differed significantly in their enzymic activity from free cells: 20alpha- and 20beta-hydroxysteroid dehydrogenase activites were substantially decreased and they also showed the capacity to synthesize significant quantities of a new derivative and to destruct steroids. Immobolized protein fractions maintained 20alpha-hydroxysteroid dehydrogenase and 20beta-hydroxysteroid dehydrogenase activities but their specific activites were lower than those of native protein fractions. It is suggested that the synthesis of new compounds was induced by acrylamide that was bound during polymerization.
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
April 1956,
Archives of biochemistry and biophysics,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
November 1969,
Journal of general microbiology,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
June 1957,
Journal of bacteriology,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
August 1968,
Journal of general microbiology,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
October 1955,
Proceedings of the National Academy of Sciences of the United States of America,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
June 2020,
Bioorganic chemistry,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
January 1990,
Annals of the New York Academy of Sciences,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
January 1973,
Acta endocrinologica. Supplementum,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
February 1974,
Steroids,
K A Koshcheenko, and
L A Krasnova, and
L K Garsiia-Rodriges, and
V I Surovtsev, and
G K Skriabin
July 2017,
Acta pharmaceutica Sinica. B,
