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Serum-free conditioned medium of human diploid fibroblast cells contains an activity that stimulates prostacyclin production by cultured bovine aortic endothelial cells. 1993
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Serum-free culture medium conditioned by human diploid fibroblast cells stimulated prostacyclin production by cultured bovine aortic endothelial cells. Gel filtration chromatography using Sephacryl S-100HR revealed at least three peaks of prostacyclin-stimulating activity. This factor was relatively heat-stable, acid-labile, trypsin-sensitive and bound to heparin Sepharose CL-6B. This factor was completely inhibited by indomethacin.
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