BIOMAT Database Logo BIOMAT Database Logo

Counterimmunoelectrophoresis in the detection of antibodies against Coccidioides immitis. 1976

F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel

In recent years counterimmunoelectrophoresis has been increasingly used in the diagnosis of microbial diseases, and our study was undertaken to evaluate its possible use in the rapid detection of antibodies against Coccidioides immitis. One hundred twelve specimens from 50 patients were studied. The study population comprised the following: Group I, 34 patients with an active coccidioidomycosis infection; Group II, five patients with a positive coccidioidin skin test; Group III, five patients with a negative coccidioidin skin test; and Group IV, six patients with other fungal infections. Counterimmunoelectrophoresis was positive in 93 of 96 specimens in Group I and negative in the other groups. The results obtained by counterimmunoelectrophoresis correlated well with complement fixation and double immunodiffusion and indicate that it is an excellent tool for the diagnosis of patients with coccidioidomycosis.

UI MeSH Term Description Entries
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D008297 Male Males
D002908 Chronic Disease Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care (Dictionary of Health Services Management, 2d ed). For epidemiological studies chronic disease often includes HEART DISEASES; STROKE; CANCER; and diabetes (DIABETES MELLITUS, TYPE 2). Chronic Condition,Chronic Illness,Chronically Ill,Chronic Conditions,Chronic Diseases,Chronic Illnesses,Condition, Chronic,Disease, Chronic,Illness, Chronic
D003045 Coccidioides A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS. Coccidioides immitis
D003047 Coccidioidomycosis Infection with a fungus of the genus COCCIDIOIDES, endemic to the SOUTHWESTERN UNITED STATES. It is sometimes called valley fever but should not be confused with RIFT VALLEY FEVER. Infection is caused by inhalation of airborne, fungal particles known as arthroconidia, a form of FUNGAL SPORES. A primary form is an acute, benign, self-limited respiratory infection. A secondary form is a virulent, severe, chronic, progressive granulomatous disease with systemic involvement. It can be detected by use of COCCIDIOIDIN. Coccidioides Infection,Coccidioides posadasii Infection,Coccidioidomycosis Infection,Coccidioides immitis Infection,San Joaquin Valley Fever,Valley Fever,Coccidioides Infections,Coccidioides immitis Infections,Coccidioides posadasii Infections,Coccidioidomycoses,Coccidioidomycosis Infections,Infection, Coccidioides,Infection, Coccidioides immitis,Infection, Coccidioides posadasii,Infection, Coccidioidomycosis,Valley Fevers
D003168 Complement Fixation Tests Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1. Complement Absorption Test, Conglutinating,Conglutination Reaction,Conglutinating Complement Absorption Test,Complement Fixation Test,Conglutination Reactions,Fixation Test, Complement,Fixation Tests, Complement,Reaction, Conglutination,Reactions, Conglutination,Test, Complement Fixation,Tests, Complement Fixation
D003378 Counterimmunoelectrophoresis Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow. Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D005500 Follow-Up Studies Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease. Followup Studies,Follow Up Studies,Follow-Up Study,Followup Study,Studies, Follow-Up,Studies, Followup,Study, Follow-Up,Study, Followup
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion

Related Publications

F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
Seroprevalence of antibodies against Coccidioides immitis in healthy horses.
June 2005, Journal of the American Veterinary Medical Association,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
Detection of antibodies to Coccidioides immitis by enzyme immunoassay.
February 1997, American journal of clinical pathology,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
The detection of Coccidioides immitis in pulmonary cytology.
January 1968, Acta cytologica,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
Inhibiting concentration of chloromycetin against Coccidioides immitis.
July 1950, Annals of western medicine and surgery,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
Effectiveness of various compounds against Coccidioides immitis.
September 1954, Antibiotics & chemotherapy (Northfield, Ill.),
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
The detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with Dirofilaria immitis antigens.
March 1976, Journal of helminthology,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
[Detection of Coccidioides immitis infection in Coahuila, Mexico].
January 2005, Revista Argentina de microbiologia,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
Fluorescent antibody inhibition test for Coccidioides immitis antibodies.
June 1966, Sabouraudia,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
Coccidioides immitis antigen.
July 1999, The Journal of infectious diseases,
F G Aguilar-Torres, and L J Jackson, and J E Ferstenfeld, and D Pappagianis, and M W Rytel
[Detection of precipitating antibodies against dermatophytes by means of counterimmunoelectrophoresis].
June 1978, Dermatologische Monatschrift,
Copied contents to your clipboard!