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Cytoplasmic RNA from imaginal discs of Drosophila melanogaster, labeled by uridine incorporation in organ culture, has been assayed by hybridization to cytological preparations of polytene chromosomes. RNA labeled during the early stages (first four hours) of ecdysone stimulation was compared to RNA labeled in the absence of the hormone. For the poly(A)-containing fraction (oligo-dT bound), several loci hybridize only RNA labeled in the presence of ecdysone; one locus hybridizes only control RNA. The majority of hybridizing loci are unaffected by the hormone. Of the loci hybridizing RNA not bound to oligo-dT, several appear specific for the ecdysone-treated sample, though most are labeled more heavily with this RNA than with the control. None of the ecdysone-sensitive loci visualized by in situ hybridization are the sites of salivary gland puffs induced by ecdysone on the same time scale.
J J Bonner, and
M L Pardue
April 1975,
Developmental biology,
J J Bonner, and
M L Pardue
January 1968,
Molecular & general genetics : MGG,
J J Bonner, and
M L Pardue
December 1974,
Developmental biology,
J J Bonner, and
M L Pardue
December 1974,
Developmental biology,
J J Bonner, and
M L Pardue
December 1977,
Cell,
J J Bonner, and
M L Pardue
August 1967,
Experimental cell research,
J J Bonner, and
M L Pardue
October 1974,
Cell,
J J Bonner, and
M L Pardue
January 1971,
Molecular & general genetics : MGG,
J J Bonner, and
M L Pardue
September 1971,
Journal of insect physiology,
J J Bonner, and
M L Pardue
December 1972,
Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen,
