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Seven of 35 male Wistar rats developed well-differentiated adenocarcinoma of the glandular stomach on combined treatment with N-methyl-N'-nitro-N-nitro soguanidine and 4-nitroquinolin 1-oxide. One of the tumors was successfully transplanted into newborn Wistar rats by subcutaneous inoculation. The latent period after inoculation was less than one month and the growth of the transplanted tumor was slow throughout 10 transplant generations. The tumor appeared nodular or cystic in subcutaneous tissues of rats and often caused ulceration of the skin. The histology of transplanted tumors was very similar to that of the primary tumor. Metastasis to both lungs was observed in one rat of the first transplant generation.
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