Biomaterial Database

Anthranilate synthase-amidotransferase (combined). A novel from of antrhanilate synthase from Euglena gracilis. 1976

C N Hankins, and S E Mills

Anthranilate synthase from Euglena gracilis strain Z was purified to electrophoretic homogeneity. The enzyme migrated as a single symmetrical peak on sucrose density gradients with a sedimentation constant of about 5.0 S. The molecular weight determined by gel filtration on Sephadex G-200 was 80,000 +/- 2,500. Polyacrylamide gel electrophoresis of the pure enzyme after sodium dodecyl sulfate denaturation gave a single band at a position corresponding to a molecular weight of 79,000 +/- 2,000. These results suggest that Euglena antranilate synthase is composed of a single polypeptide chain of about 80,000 that possesses both ammonia- and glutamine-dependent activity. All other known glutamine-dependent anthranilate synthases are heterodimers or heterotetramers with the chorismic acid and glutamine binding sites on distinct polypeptide chains.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D005056	Euglena gracilis	A species of fresh-water, flagellated EUKARYOTES in the phylum EUGLENIDA.	Euglena gracili,gracilis, Euglena
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000878	Anthranilate Synthase	An enzyme that catalyzes the formation of anthranilate (o-aminobenzoate) and pyruvic acid from chorismate and glutamine. Anthranilate is the biosynthetic precursor of tryptophan and numerous secondary metabolites, including inducible plant defense compounds. EC 4.1.3.27.	Anthranilate Synthetase,Anthranilate Synthase Component I,Synthase, Anthranilate,Synthetase, Anthranilate
D046911	Macromolecular Substances	Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.	Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular