Biomaterial Database

Dot immunoassay with biotinylated antigen for determination of antibodies against the circulating cathodic antigen (CCA) in schistosomiasis japonica. 1993

Z L Qian, and A M Deelder

Laboratory of Parasitology, Medical Faculty, University of Leiden, The Netherlands.

Based on the fact that schistosomiasis patients in both the acute and chronic phase of the infection show a strong humoral immune response against the gut-associated circulating cathodic antigen, a simple and sensitive dot immunobinding assay for schistosomiasis japonica was developed. Circulating cathodic antigen that had been purified by immunoadsorption using monoclonal antibody was biotinylated with biotin aminocaproylhydrazide via the carbohydrate moiety of the antigen. Serum samples dotted onto nitrocellulose strips were then tested in an assay involving a combined incubation step of biotinylated antigen and streptavidin peroxidase, and a subsequent staining; the total assay time was 1.5 hr. Assaying the sera of 105 uninfected controls and 104 Schistosoma japonicum-infected individuals showed a specificity of 99.0%, and sensitivities of 96.2% (acute infections) and 94.1% (chronic infections). The described assay is economic, rapid, and reproducible and lends itself to use under field conditions.

UI	MeSH Term	Description	Entries
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000909	Antibodies, Helminth	Immunoglobulins produced in a response to HELMINTH ANTIGENS.	Helminth Antibodies
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000947	Antigens, Helminth	Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.	Helminth Antigens
D001710	Biotin	A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.	Vitamin H,Biodermatin,Biokur,Biotin Gelfert,Biotin Hermes,Biotin-Ratiopharm,Biotine Roche,Deacura,Gabunat,Medebiotin,Medobiotin,Rombellin,Biotin Ratiopharm,Gelfert, Biotin,Hermes, Biotin,Roche, Biotine
D012549	Schistosoma japonicum	A species of trematode blood flukes belonging to the family Schistosomatidae whose distribution is confined to areas of the ASIA, EASTERN. The intermediate host is a snail. It occurs in man and other mammals.	Schistosoma japonicums,japonicum, Schistosoma
D012554	Schistosomiasis japonica	Schistosomiasis caused by Schistosoma japonicum. It is endemic in the ASIA, EASTERN and affects the bowel, liver, and spleen.	Schistosoma japonicum Infection,Schistosomiasis japonicum,Infection, Schistosoma japonicum,Infections, Schistosoma japonicum,Schistosoma japonicum Infections
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D015151	Immunoblotting	Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.	Dot Immunoblotting,Electroimmunoblotting,Immunoelectroblotting,Reverse Immunoblotting,Immunoblotting, Dot,Immunoblotting, Reverse,Dot Immunoblottings,Electroimmunoblottings,Immunoblottings,Immunoblottings, Dot,Immunoblottings, Reverse,Immunoelectroblottings,Reverse Immunoblottings