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In order to define the antigens recognized by cytolytic T lymphocytes (CTLs) on autologous tumors, we derived tumor-specific CTL clones from autologous mixed lymphocyte tumor cell cultures. The gene coding for a tumor rejection antigen expressed on a melanoma was isolated by transfecting genomic DNA of the tumor into an antigen-loss variant of the melanoma. Transfectants were identified on the basis of their ability to stimulate tumor necrosis factor release by the CTL clone. The gene that transferred the expression of the antigen was named MAGE-1. It is a new gene, silent in normal tissues with the exception of testis, but expressed in several types of tumors. The antigen recognized by the CTL clone is a nonapeptide derived from the protein encoded by gene MAGE-1, and presented by the HLA class I molecule HLA-A1. Using two other antimelanoma CTL clones, we identified the tyrosinase gene as coding for an antigen presented by HLA-A2 on this type of tumors. The identification of these tumor rejection antigens open new possibilities for the specific immunotherapy of cancer.
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
June 1995,
Immunological reviews,
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
June 1997,
Methods (San Diego, Calif.),
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
January 1997,
Eye (London, England),
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
May 1993,
International journal of cancer,
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
March 1996,
The Journal of experimental medicine,
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
July 1995,
Stem cells (Dayton, Ohio),
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
January 1987,
Cell,
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
January 2019,
Methods in molecular biology (Clifton, N.J.),
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
January 1994,
Annual review of immunology,
P G Coulie, and
P Weynants, and
F Lehmann, and
J Herman, and
V Brichard, and
T Wölfel, and
A Van Pel, and
E De Plaen, and
F Brasseur, and
T Boon
January 1997,
International journal of clinical & laboratory research,
