-transparent.png){kind=logo}
A detailed study of the conditions for purification of plasma factor XIII was undertaken and an optimal procedure developed. This procedure involves adsorption with aluminum hydroxide, repeated fractionation with ammonium sulfate, heat denaturation of fibrinogen, precipitation with polyethylene glycol, cryoprecipitation, and chromatography on DEAE-cellulose with gradient elution. The specific activity of the final product, as measured by the fluorescent amine incorporation assay for factor XIII, averages about 3,000-4,000 mumol dansylcadaverine incorporation/mg protein. The yield is about 20%. In SDS electrophoresis the final product has two bands under nonreducing conditions, characteristic of the a and b subunits.
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
May 1973,
Blood,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
May 1981,
The Journal of laboratory and clinical medicine,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
February 1978,
The Journal of experimental medicine,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
February 1990,
Biochemistry,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
May 1969,
The Journal of clinical investigation,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
January 1992,
The International journal of biochemistry,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
December 1980,
The Biochemical journal,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
April 1994,
Protein expression and purification,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
January 1984,
Neurochemistry international,
M Kazama, and
J McDonagh, and
R H Wagner, and
R D Langdell, and
R P McDonagh
October 1966,
Nature,
