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We have developed a simple method for the isolation of highly purified cervine (c) and ovine (o) insulin-like growth factors-I (IGF-I) and -II. The IGFs were isolated from acidified serum by cation exchange chromatography and then purified by gel filtration, chromatofocusing, and reverse-phase chromatography. The IGF preparations are > 95% pure. The cIGF-I preparation contains < 0.056% cIGF-II and the oIGF-I preparation contains < 0.01% oIGF-II. Both the IGF-II preparations contain < 0.01% IGF-I. The amino acid sequence of cIGF-I has two differences when compared with human (h) IGF-I. The cIGF-II sequence, which is identical to bovine IGF-II, has three differences when compared with hIGF-II.
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
July 1989,
Biochimica et biophysica acta,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
March 1989,
Endocrinology,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
January 1984,
Preparative biochemistry,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
July 1990,
European journal of biochemistry,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
January 1994,
The Journal of endocrinology,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
August 1985,
General and comparative endocrinology,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
September 1986,
The Journal of pediatrics,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
February 1999,
The Journal of dairy research,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
January 1990,
Therapeutische Umschau. Revue therapeutique,
L G Moore, and
D Jones, and
M A Lymburn, and
S C Hodgkinson, and
S R Davis, and
J M Suttie, and
M Sadighi, and
A Carne
December 1999,
Nihon rinsho. Japanese journal of clinical medicine,
