Biomaterial Database

Sympathetic innervation of the surface of the mouse vas deferens. 1993

N A Lavidis, and M R Bennett

Department of Physiology, University of Sydney, N.S.W., Australia.

The distribution of axons and axon varicosities on the surface of the mouse vas deferens has been determined following fluorescence of these structures with 3,3-diethyloxardicarbocyanide (DiOC2 (5)) to locate varicosities and FAGLU to detect catecholamine containing nerves. Small bundles of fluorescent axons treated with DiOC2(5), were shown to give rise to single or very small bundles of 2 or 3 varicose axons that passed over the surface of the muscle bundles. Varicosities had the same average diameter of 0.9 microns, length of 1.1 micron and spacing apart of 4.6 microns whether identified following DiOC2(5) fluorescence or the FAGLU method for catecholamines and this was shown statistically to imply that they came from the same population of varicosities. Serial thin sections through small axon bundles and single axons, viewed with the electron microscope, confirmed the dimensions of varicosities along axons observed following DiOC2(5) staining or use of the FAGLU method.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D002232	Carbocyanines	Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.	Carbocyanine
D002395	Catecholamines	A general class of ortho-dihydroxyphenylalkylamines derived from TYROSINE.	Catecholamine,Sympathin,Sympathins
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D005565	Formiminoglutamic Acid	Measurement of this acid in the urine after oral administration of histidine provides the basis for the diagnostic test of folic acid deficiency and of megaloblastic anemia of pregnancy.	FIGLU,Acid, Formiminoglutamic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001369	Axons	Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.	Axon