Biomaterial Database

[Damage to cultured corneal endothelium caused by ultrasound during phacoemulsification]. 1993

K Ogino, and F Koda, and K Miyata

Eye Clinic, Showa General Hospital, Kodaira, Japan.

An experimental study was performed to estimate the degree of corneal endothelial damage caused by ultrasound during phacoemulsification. Cultured corneal endothelium prepared from bovine eyes was exposed to ultrasound. Dead cell rates were determined by counting the number of cells positively stained by trypan blue under various conditions of ultrasonic radiation. Dead cell rate increased from 13 +/- 2% to 39 +/- 4% (n = 5, Mean +/- S.D.) as ultrasonic duration was prolonged from 15 seconds to 45 seconds. Dead cell rate increased from 6 +/- 1% to 39 +/- 4% as ultrasonic power increased from 60% to 100%. Dead cell rate increased from 8 +/- 1% to 44 +/- 11% as the distance of the phaco tip decreased from 7 mm to 4 mm. Dead cell rate increased from 4 +/- 2% to 35 +/- 7% as the angle of the phaco tip increased from 30 degrees to 90 degrees. We confirmed that endothelial damage depended on ultrasonic duration, ultrasonic power, the distance of the phaco tip, and the angle of the phaco tip. We suggest that the endothelium is damaged mainly by shock waves emanating from the phaco tip.

UI	MeSH Term	Description	Entries
D002387	Cataract Extraction	The removal of a cataractous CRYSTALLINE LENS from the eye.	Enzymatic Zonulolysis,Phakectomy,Cataract Extractions,Enzymatic Zonulolyses,Extraction, Cataract,Extractions, Cataract,Phakectomies,Zonulolyses, Enzymatic,Zonulolysis, Enzymatic
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003315	Cornea	The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)	Corneas
D004727	Endothelium	A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.	Endotheliums
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016923	Cell Death	The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.	Death, Cell