Biomaterial Database

[Scanning electron microscopic observation of hydrodissected human lens nucleus]. 1993

M Ayaki, and Y Ishii

Department of Ophthalmology, Keio University, School of Medicine, Shinjuku-ku, Japan.

Using a scanning electron microscope, we studied the surface structure of 5 different central nuclei, isolated by repeated hydrodissection during extracapsular cataract extraction. Aside from the damage induced by surgical maneuvers, the surfaces were very smooth, and there was no major disruption of the lamellar structure of the lens. Our studies have shown that hydrodissection, which allows atraumatic separation of the lens fiber layers, can be used to analyze the lamellar structure of the lens. Some of the evaluated specimens showed a distinct suture line and the lens fibers were approximately at right angles to it. These observations were different from those reported previously.

UI	MeSH Term	Description	Entries
D007907	Lens Nucleus, Crystalline	The core of the crystalline lens, surrounded by the cortex.	Crystalline Lens Nucleus,Nucleus, Crystalline Lens
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D002387	Cataract Extraction	The removal of a cataractous CRYSTALLINE LENS from the eye.	Enzymatic Zonulolysis,Phakectomy,Cataract Extractions,Enzymatic Zonulolyses,Extraction, Cataract,Extractions, Cataract,Phakectomies,Zonulolyses, Enzymatic,Zonulolysis, Enzymatic
D004210	Dissection	The separation and isolation of tissues for surgical purposes, or for the analysis or study of their structures.	Dissections
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368	Aged	A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available.	Elderly