Biomaterial Database

Further studies of 31 temperature-sensitive mutants of mouse cytomegalovirus: thermal stability, replication and analysis of temperature-sensitive functions by temperature shift. 1993

H M Akel, and A M Furarah, and C Sweet

School of Biological Sciences, Microbial Molecular Genetics and Cell Biology Research Group, University of Birmingham, Edgbaston, UK.

A study of 31 temperature-sensitive mutants of mouse cytomegalovirus has indicated that two mutants (tsm1, tsm31) may be defective in immediate-early/early functions, two (tsm2, tsm3) may be defective in early functions and six (tsm9, tsm18, tsm22, tsm23, tsm28, tsm30) may be defective in early/late functions while the remainder are late function-defective mutants as determined by temperature-shift experiments. Three mutants (tsm1, tsm2, tsm3) were more thermostable than wild-type virus while three (tsm16, tsm26, tsm28) were more thermolabile; the remainder were similar in their thermostability to wild-type virus.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D014779	Virus Replication	The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.	Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D018146	Muromegalovirus	A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, causing infection involving several organs in mice and rats. Murid herpesvirus is the type species.	Cytomegalovirus, Murine,Herpesvirus 1, Murid,Herpesvirus 2, Murid,Mouse Cytomegalovirus,Murine Cytomegalovirus,Rat Cytomegalovirus,Herpesvirus 1 (beta), Murid,Murid Herpesvirus 1,Murid Herpesvirus 2,Cytomegalovirus, Mouse,Cytomegalovirus, Rat,Cytomegaloviruses, Mouse,Cytomegaloviruses, Murine,Cytomegaloviruses, Rat,Mouse Cytomegaloviruses,Murine Cytomegaloviruses,Muromegaloviruses,Rat Cytomegaloviruses