-transparent.png){kind=logo}
Alpha-1 acid glycoprotein, also named orosomucoid (ORM), is a serum protein with genetic polymorphism. This paper describes a two-step procedure for purifying ORM from human sera. The procedure consists of both anion exchange chromatography on a DEAE-Sephadex-A50 column and affinity chromatography on a Reactive Blue 2-Sepharose CL-6B column. The purified ORM identified by polyacrylamide gel electrophoresis, SDS-polyacrylamide gradient gel electrophoresis, immunoelectrophoresis and immunodiffusion was homogeneous and reacted specifically with a commercial anti-ORM serum (Sigma). The molecular weight of the purified ORM was about 41 kd.
Related Publications
Y Hou, and
Q Gou, and
M Wu
January 1971,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
Y Hou, and
Q Gou, and
M Wu
January 1974,
Preparative biochemistry,
Y Hou, and
Q Gou, and
M Wu
October 1969,
Seikagaku. The Journal of Japanese Biochemical Society,
Y Hou, and
Q Gou, and
M Wu
June 1985,
Journal of chromatography,
Y Hou, and
Q Gou, and
M Wu
March 1984,
FEBS letters,
Y Hou, and
Q Gou, and
M Wu
February 1970,
Analytical biochemistry,
Y Hou, and
Q Gou, and
M Wu
January 2011,
Journal of biomedicine & biotechnology,
Y Hou, and
Q Gou, and
M Wu
February 1970,
The Journal of biological chemistry,
Y Hou, and
Q Gou, and
M Wu
May 1985,
Analytical biochemistry,
Y Hou, and
Q Gou, and
M Wu
April 1987,
Nihon juigaku zasshi. The Japanese journal of veterinary science,
