Studied was the antagonistic action of 4 strains of Pseudomonas aeruginosa toward 48 strains of Pasteurella multocida. Use was made of the method of the delayed antagonism after Fredericq. Ps. aeruginosa organisms were found to manifest a strongly expressed antagonistic activity as regards P. multocida. All Pasteurella strains proved highly sensitive, and showed a zone of inhibited growth sizing from 20 to 70 mm. The antibiotic substance produced by Pseudomonas aeruginosa organisms was yielded better on solid nutrient media than in liquid ones. All Pseudomonas aeruginosa strains manifested bactericidal and not bacteriostatic action upon the studied strains of Pasteurella multocida.