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The gene (aprII) encoding alkaline serine protease (AprII; subtilase) from Alteromonas sp. strain O-7 was cloned in plasmid pUC19 and transformed into Escherichia coli JM109. The nucleotide (nt) sequence of aprII has been determined. A single open reading frame (ORF) encoded a protein consisting of 621 amino acids (aa) with a M(r) of 63,958. The results of aa sequence analysis indicated that AprII is produced as a large precursor consisting of four domains: the signal sequence, the N-terminal pro-region (AprII-N), the mature AprII (AprII-M) and the C-terminal pro-region (AprII-C). The aa sequence of AprII-M shows high sequence homology with those of class-II subtilases. Two conserved sequences were found in AprII-N which might play a critical role in the maintenance of chaperone-like activity. Repeated aa sequences were observed in AprII-C (AprII-C1 and AprII-C2). The aa sequences of AprII-C1 and AprII-C2 show high sequence homology with those of the C-terminal pro-region of the other known proteases.
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
January 2019,
Microbiology resource announcements,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
October 1995,
Bioscience, biotechnology, and biochemistry,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
January 1995,
Gene,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
September 2009,
Canadian journal of microbiology,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
October 1997,
Carbohydrate research,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
July 1994,
Gene,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
February 1999,
Applied and environmental microbiology,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
January 2004,
DNA sequence : the journal of DNA sequencing and mapping,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
September 1992,
Bioscience, biotechnology, and biochemistry,
H Tsujibo, and
K Miyamoto, and
K Tanaka, and
M Kawai, and
K Tainaka, and
C Imada, and
Y Okami, and
Y Inamori
June 2002,
Bioscience, biotechnology, and biochemistry,
