Early embryonic angiogenesis in the chick area vasculosa. 1993

W E Allen, and D J Wilson
School of Biomedical Science, Queen's University of Belfast, UK.

The rate and pattern of growth as well as vessel ultrastructure of the area vasculosa were examined in the chick. The embryos were grown in shell-less culture after 3 d in ovo and staged according to Hamburger & Hamilton (1951) and the rate of increase in the diameter of the area vasculosa was measured. This revealed an increase in the area vasculosa diameter of 0.4 +/- 0.02 mm h-1 (n = 62) for embryos between stages 15 and 20. To determine the growth pattern of the sinus terminalis (the advancing edge of the area vasculosa), a marked length of the sinus was photographed at hourly intervals over a period of 9 h. It was found that this vessel grows by new vessels forming external to the sinus in the form of parallel plexuses, one of which then replaces the original sinus as the major route of bloodflow. Ultrastructurally the capillaries of the area vasculosa were simple tubes of endothelial cells, lacking a basement membrane. The endothelial cell cytoplasm contained only a few organelles, mainly mitochondria and rough endoplasmic reticulum. These findings indicate that the chick area vasculosa capillaries bear similar structural and growth characteristics to those associated with tumour angiogenesis and suggest that they may prove to be a useful model system for studying the factors involved in pathological angiogenesis.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D002196 Capillaries The minute vessels that connect arterioles and venules. Capillary Beds,Sinusoidal Beds,Sinusoids,Bed, Sinusoidal,Beds, Sinusoidal,Capillary,Capillary Bed,Sinusoid,Sinusoidal Bed
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014817 Vitelline Membrane The plasma membrane of the egg. Membrane, Vitelline,Membranes, Vitelline,Vitelline Membranes

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