Ultrastructural observation of epithelioid sarcoma. 1993

T Kanai, and R Irisawa, and H Kusama, and T Oh-i
Division of Dermatology, Tokyo Medical College, Kasumigaura Hospital, Ibaraki, Japan.

Epithelioid sarcoma located in the right hip-to-thigh region of a 58-year-old female was studied by light microscopy, immunohistochemistry, and electron microscopy. Light microscopy revealed both light and dark cells among the tumor cells. Round, homogeneous, eosinophilic bodies were noted extracellularly and intracellularly. Extracellular bodies were similar in size to tumor cells and were not stained by PAS or Congo red. In contrast, intercellular bodies were PAS positive and diastase resistant. Tumor cells were positive for vimentin, keratin, and, partially, for epithelial membrane antigen. Ultrastructurally, tumor cells had numerous microfilaments with tonofibril-like arrangement, lipid droplets, and reticulogranular structure in their cytoplasm. The reticulogranular structure was similar to the tonofilaments recognized in degenerative tumor cells. Oval filamentous bodies were recognized in the stroma. They appeared to be very similar to colloid bodies derived from tonofilaments of keratinocytes. It was considered that the filamentous bodies corresponded to extracellular eosinophilic bodies and showed signs of fibrous necrosis of tumor cells. Intracellular eosinophilic bodies were considered to be lipid droplets.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D005260 Female Females
D006615 Hip The projecting part on each side of the body, formed by the side of the pelvis and the top portion of the femur. Coxa,Coxas,Hips
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012509 Sarcoma A connective tissue neoplasm formed by proliferation of mesodermal cells; it is usually highly malignant. Sarcoma, Epithelioid,Sarcoma, Soft Tissue,Sarcoma, Spindle Cell,Epithelioid Sarcoma,Epithelioid Sarcomas,Sarcomas,Sarcomas, Epithelioid,Sarcomas, Soft Tissue,Sarcomas, Spindle Cell,Soft Tissue Sarcoma,Soft Tissue Sarcomas,Spindle Cell Sarcoma,Spindle Cell Sarcomas
D012983 Soft Tissue Neoplasms Neoplasms of whatever cell type or origin, occurring in the extraskeletal connective tissue framework of the body including the organs of locomotion and their various component structures, such as nerves, blood vessels, lymphatics, etc. Neoplasm, Soft Tissue,Neoplasms, Soft Tissue,Soft Tissue Neoplasm
D013848 Thigh The superior part of the lower extremity between the HIP and the KNEE. Thighs

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