Inhibition of CRIPTO expression and tumorigenicity in human colon cancer cells by antisense RNA and oligodeoxynucleotides. 1994

F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
Cattedra di Oncologia Medica, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Italy.

CRIPTO is an epidermal growth factor-related gene expressed in a majority of human colorectal tumors. To assess the role of CRIPTO in the growth control of human colon cancer, we have treated human colon carcinoma GEO and CBS cells, that possess high levels of CRIPTO, and WIDR colon cancer cells, that are negative for CRIPTO expression, with two antisense phosphorothioate oligodeoxynucleotides complementary to the 5' end of the human CRIPTO mRNA. Both antisense oligodeoxynucleotides significantly reduced endogenous CRIPTO protein levels and inhibited GEO and CBS cell growth in monolayer and in semisolid medium, whereas they did not affect WIDR cell growth. In addition, GEO, CBS and WIDR cells were infected with a recombinant retroviral vector containing the hygromycin-resistance gene and a 900 bp EcoRI-EcoRI coding fragment of the human CRIPTO cDNA oriented in the 3' to 5' direction. GEO and CBS CRIPTO antisense infectants exhibited a 60 to 70% reduction in CRIPTO protein expression, in monolayer growth and in soft agar cloning efficiency as compared to parental noninfected cells. In contrast, infection of WIDR cells with the CRIPTO antisense retrovirus did not alter their growth. Finally, GEO CRIPTO antisense infectants formed tumors in nude mice that were significantly smaller and had a larger latency period as compared to noninfected GEO cells.

UI MeSH Term Description Entries
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009363 Neoplasm Proteins Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. Proteins, Neoplasm
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003110 Colonic Neoplasms Tumors or cancer of the COLON. Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D004815 Epidermal Growth Factor A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form. EGF,Epidermal Growth Factor-Urogastrone,Urogastrone,Human Urinary Gastric Inhibitor,beta-Urogastrone,Growth Factor, Epidermal,Growth Factor-Urogastrone, Epidermal,beta Urogastrone
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
March 1991, Cancer research,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
April 1996, Proceedings of the National Academy of Sciences of the United States of America,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
February 1994, Journal of leukocyte biology,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
April 2000, European journal of cancer (Oxford, England : 1990),
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
February 1997, Nature medicine,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
October 2001, Alimentary pharmacology & therapeutics,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
April 1997, Cancer research,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
August 1997, International journal of cancer,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
August 1988, Proceedings of the National Academy of Sciences of the United States of America,
F Ciardiello, and G Tortora, and C Bianco, and M P Selvam, and F Basolo, and G Fontanini, and F Pacifico, and N Normanno, and R Brandt, and M G Persico
January 2005, Methods in molecular medicine,
Copied contents to your clipboard!