-transparent.png){kind=logo}
The binding of methotrexate to human serum albumin and the inhibitory effect of serum free fatty acids (FFA) have been studied by equilibrium dialysis with radiolabeled methotrexate. Methotrexate was bound to albumin via a single site (1.03 +/- 0.02) with a low affinity (1350 +/- 60 M-1). The effect of FFA on binding by albumin of methotrexate was analysed according to the classical inhibition models with computation of the free inhibitor concentration and was ascribed to an uncompetitive type of inhibition. These results were in agreement with the observed serum binding of methotrexate (45-50%) and allowed the simulation of the effect of various concentrations of FFA on methotrexate albumin binding in human serum.
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
January 1981,
Pharmacology,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
January 1980,
Acta pharmaceutica Suecica,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
November 1973,
Annals of the New York Academy of Sciences,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
May 1991,
International journal of clinical pharmacology, therapy, and toxicology,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
May 1975,
Journal of lipid research,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
March 1972,
Biochimica et biophysica acta,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
July 1980,
The Journal of biological chemistry,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
August 1971,
Biochemistry,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
June 2014,
Physical chemistry chemical physics : PCCP,
P Claudepierre, and
S Urien, and
O Chassany, and
J P Tillement
February 1972,
The Biochemical journal,
