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Nuclei prepared from MOPC-21 cells were treated with the nonionic detergents Triton X-100 or Nonidet P-40. Chemical analysis revealed that nearly 90% of the nuclear phospholipid was removed by detergent treatment. The membrane-denuded nuclei remained intact with preservation of nuclear pore complexes as demonstrated by electron microscopy. Ribonucleic acid transport from detergent-treated nuclei proceeded at the same rate and to the same extent as in control nuclei. Normal nuclear restriction of nucleic acids was unaltered by removal of the nuclear membranes. The effect of temperature on transport of RNA from freshly isolated myeloma nuclei with intact nuclear envelopes was studied. No temperature transition was associated with the transport process. These data indicate that the transport of macromolecules from isolated myeloma nuclei is independent of the nuclear membrane.
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
January 1981,
Cell biology international reports,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
August 1981,
Archives of biochemistry and biophysics,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
August 1973,
Cancer research,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
May 1986,
Nucleic acids research,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
March 1982,
Biokhimiia (Moscow, Russia),
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
May 1981,
Molecular biology reports,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
November 1978,
Proceedings of the National Academy of Sciences of the United States of America,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
June 1977,
Nucleic acids research,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
November 1980,
The Biochemical journal,
S E Stuart, and
G A Clawson, and
F M Rottman, and
R J Patterson
December 1978,
Biokhimiia (Moscow, Russia),
