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Construction and characterization of a YAC library with a low frequency of chimeric clones from flow-sorted human chromosome 9. 1993
M K McCormick, and
A Buckler, and
W Bruno, and
E Campbell, and
K Shera, and
D Torney, and
L Deaven, and
R Moyzis
Center for Human Genome Studies and Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Human chromosome 9 DNA, flow-sorted from somatic cell hybrid PK-87-9, has been used to construct two complete digest YAC libraries. The combined representation of chromosome 9 in these libraries, estimated by hybridization of chromosome 9-specific sequences to YAC colony grids, is approximately 95%. The frequency of chimeric clones, analyzed by fluorescence in situ hybridization of chromosome 9 YACs to human metaphase chromosomes, was estimated to be approximately 4%. These libraries provide a resource for physical mapping and for moving from genetic markers to disease loci on chromosome 9.
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