-transparent.png){kind=logo}
The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived from cloned PCR fragments demonstrated that the toxin gene encodes a protein of 1,296 amino acid residues. Comparative alignment of the derived infant BoNT/A sequence with those of other published neurotoxins revealed highest sequence relatedness with BoNT/A of classical food-borne botulism. The sequence identity between infant and classical BoNT/A was 94.9% for the light chain (corresponding to 23 amino acid changes) and 87.1% for the heavy chain (corresponding to 109 amino acid changes).
A Willems, and
A K East, and
P A Lawson, and
M D Collins
December 1993,
Biochimica et biophysica acta,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
February 1994,
Current microbiology,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
April 1993,
FEMS microbiology letters,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
June 1990,
The Journal of biological chemistry,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
October 1998,
Infection and immunity,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
January 1995,
Microbiology and immunology,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
July 2006,
Archives of toxicology,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
January 2003,
Biochimica et biophysica acta,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
February 2014,
Epidemiology and infection,
A Willems, and
A K East, and
P A Lawson, and
M D Collins
November 2008,
BMC evolutionary biology,
