Both 17 beta-estradiol (E2) and testosterone (T) were shown to inhibit in vitro pituitary LH secretion in the turtle Trachemys scripta. Since T was approximately 500 times less potent than E2, and 5 alpha-dihydrotestosterone was even less active than T, the inhibitory action of T may result from its aromatization to estrogen. We utilized both in vivo and in vitro approaches to elucidate the roles of T and estrogen in the negative feedback of pituitary LH secretion. Gonadectomy of adult (vitellogenic) females significantly elevated plasma LH. Adult females treated with fadrozole (an aromatase inhibitor) with or without daily injections of keoxifene (an antiestrogen) also showed an increase in plasma LH to a level comparable to that observed in gonadectomized females, whereas plasma LH levels of juvenile females treated with fadrozole remained undetectable. In vitro LH secretion in response to GnRH in juvenile females was significantly inhibited by 48-h exposure to 50 ng/ml T or 100 pg/ml E2. Both fadrozole (200 microM) and keoxifene (200 nM) significantly blocked this T-induced inhibition of LH secretion, demonstrating that T lacks intrinsic inhibitory activity. Confirmation of the inhibition of aromatase activity by fadrozole comes from metabolic studies of 1 beta-[3H]androstenedione using turtle brain, ovary, and pituitary. In vitro, fadrozole altered the metabolism of 1 beta-[3H]androstenedione and inhibited aromatase activities in these tissues. These results indicate that the inhibitory effect of T is largely mediated through its aromatization to estrogen, and that estrogen is primarily responsible for the suppressed LH secretion in vitellogenic adult turtles.