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The effect of inorganic orthophosphate (Pi) on iron-induced lipid peroxidation in non-respiring rat liver mitochondria was studied and compared with that of adenosine 5'-diphosphate (ADP) at pH 7.4. Under the experimental conditions used, lipid peroxidation was induced by Fe2+ in conjunction with Pi and/or ADP, but not by Fe2+ alone. The characteristics of Fe(2+)-induced lipid peroxidation supported by Pi and by ADP were different: ADP-supported peroxidation took place after a lag-time and proceeded until all the O2 in the incubation medium was consumed, whereas the Pi-supported peroxidation exhibited a longer lag-time and a higher rate, but stopped when half the O2 was consumed. These features were ascribed to the fact that Pi forms active iron-oxygen complexes more rapidly than ADP, but the lifetimes of these complexes are less than those of the complexes with ADP. Furthermore, the ability of Pi to form active iron-oxygen complexes was found to be less than that of ADP. Fe2+, in conjunction with Pi and/or ADP, was found to induce lipid peroxidation in respiring mitochondria, although to a lesser extent than in non-respiring mitochondria.
H Kawano, and
K Kogure, and
K Fukuzawa, and
H Terada
October 1974,
Acta medica Okayama,
H Kawano, and
K Kogure, and
K Fukuzawa, and
H Terada
June 1963,
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H Kawano, and
K Kogure, and
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H Terada
March 1998,
Cell biochemistry and function,
H Kawano, and
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K Fukuzawa, and
H Terada
August 1996,
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H Kawano, and
K Kogure, and
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H Terada
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H Kawano, and
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H Kawano, and
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H Terada
December 1977,
Chemico-biological interactions,
H Kawano, and
K Kogure, and
K Fukuzawa, and
H Terada
January 2001,
Ukrains'kyi biokhimichnyi zhurnal (1999 ),
H Kawano, and
K Kogure, and
K Fukuzawa, and
H Terada
January 1982,
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H Kawano, and
K Kogure, and
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January 1986,
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