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We compared the effects of specimen turbidity and glycerol concentration on nine enzymatic methods for triglyceride measurement. We assayed 51 specimens with triglyceride concentrations of 0.85-8.21 mmol/L (75-727 mg/dL) and turbidity at 420 nm equivalent to > or = 0.1 mmol/L (8.8 mg/dL) triglyceride (measured as part of our comparison method). The data were analyzed by multiple regression, which gave coefficients for the effects of glycerol concentration and the change in turbidity during the reaction. The effects of specimen turbidity and glycerol concentration were method-dependent and ranged from 6.20% to -15.67% of the measured result. The magnitude of the turbidity effect (in assays with a significant turbidity interference) was similar to that for glycerol (in assays with a significant glycerol interference). A triglyceride assay with a bichromatic measurement was less subject to interference from turbidity.
M Sampson, and
M Ruddel, and
R J Elin
April 1970,
Medizinische Klinik,
M Sampson, and
M Ruddel, and
R J Elin
November 1968,
Journal of lipid research,
M Sampson, and
M Ruddel, and
R J Elin
October 1977,
Das Medizinische Laboratorium,
M Sampson, and
M Ruddel, and
R J Elin
January 1995,
JPEN. Journal of parenteral and enteral nutrition,
M Sampson, and
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June 1968,
Zeitschrift fur klinische Chemie und klinische Biochemie,
M Sampson, and
M Ruddel, and
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January 2006,
Clinical chemistry and laboratory medicine,
M Sampson, and
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May 1992,
Clinical chemistry,
M Sampson, and
M Ruddel, and
R J Elin
March 1966,
Clinica chimica acta; international journal of clinical chemistry,
M Sampson, and
M Ruddel, and
R J Elin
December 1969,
Schweizerische medizinische Wochenschrift,
M Sampson, and
M Ruddel, and
R J Elin
March 2000,
Journal of UOEH,
