BIOMAT Database Logo BIOMAT Database Logo

The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb. 1994

R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
Department of Cell Biology, School of Medicine, Vanderbilt University, Nashville, TN 37232.

The Schizosaccharomyces pombe cdc5+ gene was identified in the first screen for cell division cycle mutants in this yeast. The cdc5+ gene was reported to be required for nuclear division but because of its modest elongation and leaky nature at the non-permissive temperature, it was not investigated further. Here, we report the characterization of the single allele of this gene, cdc5-120, in more detail. The mutant arrests with a 2N DNA content and a single interphase nucleus. Further genetic analyses suggest that cdc5+ gene function is essential in the G2 phase of the cell cycle. We have cloned and sequenced the cdc5+ gene. The deduced protein sequence predicts that Cdc5 is an 87 kDa protein and contains a region sharing significant homology with the DNA binding domain of the Myb family of transcription factors. Deletion mapping of the cdc5+ gene has shown that the N-terminal 232 amino acids of the protein, which contain the Myb-related region, are sufficient to complement the cdc5ts strain. A cdc5 null mutant was generated by homologous recombination. Haploid cells lacking cdc5+ are inviable, indicating that cdc5+ is an essential gene. A fusion protein consisting of bacterial glutathione S-transferase joined in-frame to the N-terminal 127 amino acids of the Cdc5 protein is able to bind to DNA cellulose at low salt concentrations. This evidence suggests that cdc5+ might encode a transcription factor whose activity is required for cell cycle progression and growth during G2.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D011518 Proto-Oncogene Proteins Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity. Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004261 DNA Replication The process by which a DNA molecule is duplicated. Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D004271 DNA, Fungal Deoxyribonucleic acid that makes up the genetic material of fungi. Fungal DNA
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D005800 Genes, Fungal The functional hereditary units of FUNGI. Fungal Genes,Fungal Gene,Gene, Fungal
D005816 Genetic Complementation Test A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell. Allelism Test,Cis Test,Cis-Trans Test,Complementation Test,Trans Test,Allelism Tests,Cis Tests,Cis Trans Test,Cis-Trans Tests,Complementation Test, Genetic,Complementation Tests,Complementation Tests, Genetic,Genetic Complementation Tests,Trans Tests

Related Publications

R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis.
August 1995, The Journal of cell biology,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
A Schizosaccharomyces pombe gene that promotes sexual differentiation encodes a helix-loop-helix protein with homology to MyoD.
January 1993, The EMBO journal,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis.
June 1994, The Journal of cell biology,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
Identification of act2, an essential gene in the fission yeast Schizosaccharomyces pombe that encodes a protein related to actin.
January 1992, Proceedings of the National Academy of Sciences of the United States of America,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
Vaccinia virus encodes an essential gene with strong homology to protein kinases.
December 1989, The Journal of biological chemistry,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
The novel gene trs1 encodes an essential protein for the transition from mitotic cell cycle to resting state in Schizosaccharomyces pombe.
November 1991, FEMS microbiology letters,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
The ste4+ gene, essential for sexual differentiation of Schizosaccharomyces pombe, encodes a protein with a leucine zipper motif.
December 1991, Nucleic acids research,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein.
December 1991, The EMBO journal,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
The Schizosaccharomyces pombe cho1+ gene encodes a phospholipid methyltransferase.
October 1998, Genetics,
R Ohi, and D McCollum, and B Hirani, and G J Den Haese, and X Zhang, and J D Burke, and K Turner, and K L Gould
The Schizosaccharomyces pombe gms1+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation.
March 1997, Biochemical and biophysical research communications,
Copied contents to your clipboard!