Biomaterial Database

Sperm selection by PD-10 sephadex columns: comparison with SpermPrep filtration and Percoll centrifugation. 1993

O López, and A Mata, and M Antich, and L Bassas

Laboratory of Andrology, Fundación Puigvert, Barcelona, Spain.

The efficacy of a disposable, prepacked column (PD-10) containing Sephadex G-25, to select motile spermatozoa, was compared with another column for sperm filtration (SpermPrep) and centrifugation through Percoll gradients. Aliquots of washed sperm suspensions were processed by the three techniques. The number of motile cells and the proportion of total spermatozoa selected was similar for all methods. Recovery of spermatozoa showing optimal movement was 145.9 +/- 30% (mean +/- SEM) with PD-10 columns and 131.9 +/- 32% with Percoll, both significantly higher than SpermPrep (71.9 +/- 11%; P < 0.05). The straight line velocity of motile cells was lower in samples processed by SpermPrep (29.3 +/- 2 microns/S) compared to both PD-10 (34.7 +/- 1 micron/s) and Percoll (34.9 +/- 2 microns/s; P = 0.07). When whole semen was processed, total sperm recovery with PD-10 was 61.7 +/- 8% versus 47.7 +/- 7% with Percoll (P < 0.001). Percoll centrifugation improved the percentage of morphologically normal spermatozoa more than PD-10. Similar proportions of motile spermatozoa and cells with optimal motility were obtained by both methods. We conclude that PD-10 filtration columns can be used to prepare semen in the laboratory as a practical alternative to other methods.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002499	Centrifugation, Density Gradient	Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013081	Sperm Motility	Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.	Motilities, Sperm,Motility, Sperm,Sperm Motilities
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms