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[Histochemical and electron microscopic study of the formation of trabecular meshwork and changes of glycosaminoglycans]. 1993

T Tanino, and T Hida, and N Azuma
Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Japan.

The development of iridocorneal angle and trabecular tissue was investigated histochemically and electron microscopically. Sixty-three human eyes at from 5 to 22 weeks of gestation were used in this study. For identifying glycosaminoglycans, alcian blue staining and enzyme digestion methods with hyaluronidase and chondroitinase AC and ABC were carried out light microscopically. Electron microscopically, specimens were stained with ruthenium red. In the early stage from 8 to 10 weeks, mesenchymal cells between the primordium of the cornea and the iris became elongated and connected with one another. Desmosome-like junctional complexes were observed in these cells. Schlemm's canal and para-canalicular tissue were observed at 20 weeks and the structure of the meshwork became similar that of adults. From findings of enzyme digestion methods, glycosaminoglycans in trabecular tissue seemed to be mainly hyaluronic acid in the early stage, which was replaced by chondroitin sulfate and dermatan sulfate afterwards. Substances positive for ruthenium red, which seemed to be glycosaminoglycans, was observed in intercellular spaces electron microscopically. They decreased according to development. These findings indicated that the beginning of aqueous outflow might be related to the decrease of glycosaminoglycans in trabecular tissue.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D005865 Gestational Age The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination. Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages
D006025 Glycosaminoglycans Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE). Glycosaminoglycan,Mucopolysaccharides
D006651 Histocytochemistry Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods. Cytochemistry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014129 Trabecular Meshwork A porelike structure surrounding the entire circumference of the anterior chamber through which aqueous humor circulates to the SCHLEMM'S CANAL. Meshwork, Trabecular,Meshworks, Trabecular,Trabecular Meshworks

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