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Osmium tetroxide in the presence of pyridine or 2,2'-bipyridine has been found to react completely with the pyrimidine moieties (thymine, uracil, and cytosine) in polynucleotides. Pyrimidine osmate ester moieties, L2OSO4-pyrimidine, were formed. The OSO4 has added across the 5,6 double bond and L=pyridine or 1/2-bipyridine. The pyridine derivatives were not stable and decomposed slowly after the OSO4-pyridine reagent was removed by gel chromatography. Labeled poly(uridylic acid) lost osmium completely during gel chromatography unless the eluent contained a high concentration of pyridine. The products formed between OSO4-bipyridine and polynucleotides were much more stable and the OS label was retained during and after gel chromatography. Both the OSO4-pyridine and OSO4-bipyridine reagents reacted more rapidly than the OSO4-CN-reagent.
C H Chang, and
M Beer, and
L G Marzilli
June 1978,
Bioinorganic chemistry,
C H Chang, and
M Beer, and
L G Marzilli
June 1967,
Biochimica et biophysica acta,
C H Chang, and
M Beer, and
L G Marzilli
April 1986,
The Biochemical journal,
C H Chang, and
M Beer, and
L G Marzilli
April 1957,
Experimental cell research,
C H Chang, and
M Beer, and
L G Marzilli
January 1963,
Zeitschrift fur mikroskopisch-anatomische Forschung,
C H Chang, and
M Beer, and
L G Marzilli
June 2007,
Inorganic chemistry,
C H Chang, and
M Beer, and
L G Marzilli
September 1989,
Biophysical chemistry,
C H Chang, and
M Beer, and
L G Marzilli
May 1968,
Photochemistry and photobiology,
C H Chang, and
M Beer, and
L G Marzilli
June 1965,
Laboratory investigation; a journal of technical methods and pathology,
C H Chang, and
M Beer, and
L G Marzilli
December 1977,
Journal of molecular biology,
