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Hemoglobin (Hb) adducts, formed by the neurotoxic agent acrylamide (AA) and its genotoxic metabolite glycidamide (GA), were measured in the rat by means of a method for simultaneous determination of the adducts formed to cysteine. A novel, nonlinear dosimetric model was developed to describe Hb adduct formation. This model incorporates the saturable kinetics of the metabolic conversion in vivo of AA to GA. The pharmacokinetic parameters Vmax and Km and the first-order rates of elimination, k1 and k2, for AA and GA from all processes except conversion of AA to GA, were estimated directly from Hb adduct data to 19 M hr-1, 66 microM, 0.21 hr-1, and 0.48 hr-1, respectively. At low concentrations, approximately 60% of AA was metabolized to GA. The nonlinear dosimetric model for adduct formation has potential general applicability in high-to-low-dose extrapolation of genotoxic effects.
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
February 2003,
Journal of toxicology and environmental health. Part A,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
February 2003,
Mutation research,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
June 2023,
Environmental science and pollution research international,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
November 2012,
International journal of cancer,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
May 2005,
Toxicological sciences : an official journal of the Society of Toxicology,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
October 2003,
Chemical research in toxicology,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
February 2008,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
November 2020,
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
February 2005,
Mutation research,
C J Calleman, and
E Bergmark, and
L G Stern, and
L G Costa
March 2023,
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology,
