-transparent.png){kind=logo}
Mice homozygous for the recessive allelic mutation motheaten (me) or viable motheaten (mev) on chromosome 6 develop severe defects in hematopoiesis. In this paper we present the findings that the me and mev mutations are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene. High resolution mapping localized me to an area tightly linked to Hcph on chromosome 6. Abnormalities of the Hcph protein product were demonstrated by Western blot analysis and by activity assays in both me/me and mev/mev mice. Molecular analysis of the Hcph cDNA identified abnormal transcripts in both mutants. DNA sequence analyses of cDNA and genomic clones revealed that both the me and mev mutations are point mutations that result in aberrant splicing of the Hcph transcript. These findings provide the first available animal models for a specific protein-tyrosine phosphatase deficiency, thus facilitating determination of the precise role of this signaling molecule in hematopoiesis.
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
January 2001,
Journal of immunology (Baltimore, Md. : 1950),
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
June 1996,
Journal of immunology (Baltimore, Md. : 1950),
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
June 1993,
Nature genetics,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
November 1992,
Genomics,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
September 1999,
Gene,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
September 2014,
Actas urologicas espanolas,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
June 1995,
The Biochemical journal,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
August 1984,
The American journal of pathology,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
May 1999,
The Journal of experimental zoology,
L D Shultz, and
P A Schweitzer, and
T V Rajan, and
T Yi, and
J N Ihle, and
R J Matthews, and
M L Thomas, and
D R Beier
July 1985,
Experimental hematology,
