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The induction of anti-influenza cytotoxic T lymphocytes (CTL) in vivo by immunizing mice with liposomes containing messenger RNA (mRNA) encoding the influenza virus nucleoprotein (NP) is described. NP mRNA, obtained by in vitro transcription, was encapsulated into simple cholesterol/phosphatidylcholine/phosphatidylserine liposomes by the detergent removal technique. The dependence of the route of mRNA-liposomes delivery on CTL induction was studied. The CTL induced were identical to those obtained in vivo with infectious virus in terms of specificity, lysing both peptide-sensitized and virus-infected targets. Furthermore, with the same mRNA-liposome preparation, virus-specific CTL responses could be also elicited in mice of three different haplotypes each of them known to present a distinct NP peptide in an MHC-restricted fashion. The relevance of these results in the context of vaccine development is discussed.
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
February 2001,
Vaccine,
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
September 1992,
Journal of immunology (Baltimore, Md. : 1950),
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
August 2003,
Blood,
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
April 2001,
Vaccine,
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
November 1991,
Journal of immunology (Baltimore, Md. : 1950),
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
October 1991,
Immunology letters,
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
September 1989,
Journal of immunology (Baltimore, Md. : 1950),
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
January 1982,
Archives of virology,
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
July 1993,
Journal of immunology (Baltimore, Md. : 1950),
F Martinon, and
S Krishnan, and
G Lenzen, and
R Magné, and
E Gomard, and
J G Guillet, and
J P Lévy, and
P Meulien
January 1994,
Virology,
