Biomaterial Database

Effect of administration of porcine follicular fluid to gilts during the luteal phase of the estrous cycle on plasma gonadotropins, follicular development, and ovulation rate. 1993

R V Knox, and D R Zimmerman

Department of Animal Science, University of Nebraska, Lincoln 68583-0908.

Eighty gilts were assigned randomly to receive twice daily i.v. injections of either 5 mL of steroid-reduced porcine follicular fluid (pFF) or .15 M saline (S) to evaluate the effects of suppression of FSH before, during, and 1 d after induced luteolysis on d 13 (d 0 = onset of estrus) on development of medium and large follicles during the follicular phase and subsequent ovulation rate. Ovaries were recovered and assessed for numbers of corpora albicantia (CA) and medium (NMF, 3 to 6.9 mm) and large (NLF, > 6.9 mm) follicles on d 13, 15, and 17 of the estrous cycle and number of corpora lutea (CL) in the group assigned to be examined after ovulation. Gilts assigned to the postovulatory group were bled at 12-h intervals from d 9.5 through 17. Administration of pFF suppressed concentrations of FSH during treatment with pFF (pFF, .38 +/- .02 vs S, .52 +/- .02 ng/mL, P < .05) and reduced NMF on d 13 (pFF, 17.3 +/- 4.0 vs S, 45.8 +/- 4.0, P < .01) and d 15 (28.1 +/- 4.0 vs 38.7 +/- 4.2, P < .05). The NMF declined markedly between d 15 and 17 in control gilts but not in pFF-treated gilts; pFF and S gilts had achieved similar NMF on d 17 (pFF, 23.1 +/- 4.0 vs S, 21.3 +/- 4.2). Large follicles were first observed on d 17 and were reduced substantially by prior treatment with pFF (pFF, 6.7 +/- 1.4 vs S, 12.4 +/- 1.5, P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D007986	Luteinizing Hormone	A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity.	ICSH (Interstitial Cell Stimulating Hormone),Interstitial Cell-Stimulating Hormone,LH (Luteinizing Hormone),Lutropin,Luteoziman,Luteozyman,Hormone, Interstitial Cell-Stimulating,Hormone, Luteinizing,Interstitial Cell Stimulating Hormone
D008183	Luteal Phase	The period in the MENSTRUAL CYCLE that follows OVULATION, characterized by the development of CORPUS LUTEUM, increase in PROGESTERONE production by the OVARY and secretion by the glandular epithelium of the ENDOMETRIUM. The luteal phase begins with ovulation and ends with the onset of MENSTRUATION.	Menstrual Cycle, Luteal Phase,Menstrual Cycle, Secretory Phase,Menstrual Secretory Phase,Postovulatory Phase,Phase, Luteal,Phase, Postovulatory,Secretory Phase, Menstrual
D010060	Ovulation	The discharge of an OVUM from a rupturing follicle in the OVARY.	Ovulations
D011897	Random Allocation	A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.	Randomization,Allocation, Random
D003008	Cloprostenol	A synthetic prostaglandin F2alpha analog. The compound has luteolytic effects and is used for the synchronization of estrus in cattle.	Cloprostenol Monosodium Salt,Cloprostenol Sodium,Estrumate,ICI-80,966,Oestrophan,ICI 80,966,ICI80,966,Monosodium Salt, Cloprostenol,Salt, Cloprostenol Monosodium,Sodium, Cloprostenol
D004971	Estrus	The period in the ESTROUS CYCLE associated with maximum sexual receptivity and fertility in non-primate female mammals.
D005260	Female		Females
D005498	Follicular Phase	The period of the MENSTRUAL CYCLE representing follicular growth, increase in ovarian estrogen (ESTROGENS) production, and epithelial proliferation of the ENDOMETRIUM. Follicular phase begins with the onset of MENSTRUATION and ends with OVULATION.	Menstrual Cycle, Follicular Phase,Menstrual Cycle, Proliferative Phase,Menstrual Proliferative Phase,Preovulatory Phase,Phase, Follicular,Phase, Menstrual Proliferative,Phase, Preovulatory,Proliferative Phase, Menstrual
D005640	Follicle Stimulating Hormone	A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Follicle-stimulating hormone stimulates GAMETOGENESIS and the supporting cells such as the ovarian GRANULOSA CELLS, the testicular SERTOLI CELLS, and LEYDIG CELLS. FSH consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity.	FSH (Follicle Stimulating Hormone),Follicle-Stimulating Hormone,Follitropin
D006080	Ovarian Follicle	An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.	Graafian Follicle,Atretic Follicle,Ovarian Follicles,Atretic Follicles,Follicle, Atretic,Follicle, Graafian,Follicle, Ovarian,Follicles, Atretic,Follicles, Graafian,Follicles, Ovarian,Graafian Follicles