Biomaterial Database

[Change in interphotoreceptor-retinoid-binding-protein (IRBP) after retinal photocoagulation and cryopexy]. 1993

Y Taniguchi

Department of Ophthalmology, Tokyo Medical College, Sinjuku-ku, Japan.

Effects of retinal photocoagulation and cryopexy on the interphotoreceptor-retinoid-binding-protein (IRBP) were studied using the indirect enzyme-labeled antibody technique. Following photocoagulation or cryopexy of eyes of normal guinea pigs, the eyes were fixed and frozen sections were reacted with rabbit anti-bovine IRBP serum as a primary antibody and with horseradish-peroxidase-conjugated anti-rabbit IgG goat IgG Fab' fragment as a secondary antibody. A positive reaction for IRBP was noted up until 7 days after photocoagulation. However, although IRBP was also noted in almost all layers of the retina immediately following cryopexy, it disappeared with time as the retina atrophied. We postulate that, because IRBP is an extracellular substance produced by the visual cell, it tended to remain in the photoreceptor layer following milder destruction with photocoagulation, but disappeared completely with atrophy of visual cells following cryopexy.

UI	MeSH Term	Description	Entries
D008028	Light Coagulation	The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)	Photocoagulation,Coagulation, Light,Coagulations, Light,Light Coagulations,Photocoagulations
D012160	Retina	The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.	Ora Serrata
D003452	Cryosurgery	The use of freezing as a special surgical technique to destroy or excise tissue.	Cryoablation,Cryoablations,Cryosurgeries
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005136	Eye Proteins	PROTEINS derived from TISSUES of the EYE.	Proteins, Eye
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012177	Retinol-Binding Proteins	Proteins which bind with RETINOL. The retinol-binding protein found in plasma has an alpha-1 mobility on electrophoresis and a molecular weight of about 21 kDa. The retinol-protein complex (MW	Retinoid Binding Protein,Retinol Binding Protein,Retinoid Binding Protein, F-Type,Retinoid Binding Proteins,Retinol Binding Proteins,Binding Protein, Retinoid,Binding Protein, Retinol,Binding Proteins, Retinoid,Binding Proteins, Retinol,Protein, Retinoid Binding,Protein, Retinol Binding,Retinoid Binding Protein, F Type