Biomaterial Database

Assay of inert gas contamination in studies of hydrostatic pressure effects. 1993

M Li, and S K Hong, and C V Paganelli, and P M Hogan

Department of Physiology, State University of New York, Buffalo 14214.

In hyperbaric tissue studies it is common to use gas pressure as a means for increasing the hydrostatic pressure of the preparation. When this is done, one must take the necessary precautions to avoid gas contamination of the tissue. In the present study, we demonstrate a) methods for calculating the gas contamination using particular materials in the perfusion system, and b) a technique for isolating the fluid environment from contamination by the compressing gas in a flow-through tissue chamber. The present work is exemplified with a specific apparatus, but the general principle is applicable to other studies.

UI	MeSH Term	Description	Entries
D009584	Nitrogen	An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D010313	Partial Pressure	The pressure that would be exerted by one component of a mixture of gases if it were present alone in a container. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Partial Pressures,Pressure, Partial,Pressures, Partial
D004242	Diving	An activity in which the organism plunges into water. It includes scuba and bell diving. Diving as natural behavior of animals goes here, as well as diving in decompression experiments with humans or animals.	Divings
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001273	Atmosphere Exposure Chambers	Experimental devices used in inhalation studies in which a person or animal is either partially or completely immersed in a chemically controlled atmosphere.	Atmosphere Exposure Chamber,Chamber, Atmosphere Exposure,Chambers, Atmosphere Exposure,Exposure Chamber, Atmosphere,Exposure Chambers, Atmosphere
D015194	Diffusion Chambers, Culture	Devices used in a technique by which cells or tissues are grown in vitro or, by implantation, in vivo within chambers permeable to diffusion of solutes across the chamber walls. The chambers are used for studies of drug effects, osmotic responses, cytogenic and immunologic phenomena, metabolism, etc., and include tissue cages.	Culture Diffusion Chambers,Semi-Permeable Chambers,Tissue Cages,Semipermeable Chambers,Cage, Tissue,Cages, Tissue,Chamber, Culture Diffusion,Chamber, Semi-Permeable,Chamber, Semipermeable,Chambers, Culture Diffusion,Chambers, Semi-Permeable,Chambers, Semipermeable,Culture Diffusion Chamber,Diffusion Chamber, Culture,Semi Permeable Chambers,Semi-Permeable Chamber,Semipermeable Chamber,Tissue Cage