Biomaterial Database

Effects of methylsulphonyl polychlorinated biphenyl homologues and 7,8-benzoflavone on aryl hydrocarbon hydroxylase activity. 1993

J Nagayama, and Y Masuda

Laboratory of Environmental Health Sciences, School of Health Sciences, Kyushu University, Fukuoka.

First, we investigated the effects of eleven methylsulphonyl polychlorinated biphenyl (MSF-PCB) homologues at 1.5 micrograms/ml and 7,8-benzoflavone (ANF) at 1.4 micrograms/ml on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 15 ng/ml)-induced aryl hydrocarbon hydroxylase (AHH) activity of cultured human lymphoblastoid cells and the following results were obtained. 1) The relative AHH activity (TCDD-induced AHH activity treated with each of the MSF-PCB compounds or ANF/the enzyme activity treated with acetone, %) of the ANF-treated culture was the smallest value (about 9%). 2) Among MSF-PCB homologues, 3-MSF-4,5,3',4'- and 4-MSF-3,5,3',4'-tetrachlorobiphenyls (TCBs) showed the highest inhibition and their relative enzyme activities were about 20%. 3) In the rest of MSF-PCB compounds, the relative AHH activities were as follows: 4-MSF-3,5,3',4',5'-pentachlorobiphenyl (PenCB); 37%, 3-MSF-4,5,2',3',4'-PenCB; 50%, 3-MSF-4,5,2',3'-TCB, 3-MSF-4,5,2',3',4',5'-hexachlorobiphenyl and 3-MSF-4,5,6,2',3',4',5'-heptachlorobiphenyl; 60 to 64%, 4-MSF-2,5,2',3',4'-PenCB; 77%, 3-MSF-2,5,3',4'-TCB; 88%, 4-MSF-2,5,2',5'-TCB; 93% and 3-MSF-4,5,3',4',5'-PenCB rather enhanced the TCDD-induced enzyme activity. Second, we prepared the hepatic microsomes of BALB/c (Ah responsive) and AKR/J (Ah nonresponsive) strains of mice after the treatment of olive oil (as control), 3-methylcholanthrene (MC, 42 mg/kg, once) and TCDD (20 micrograms/kg, 6 times, once every other week) in order to examine the effects of 3-MSF-4,5,3',4'-TCB (1.5 to 45 micrograms/ml) and ANF (1.4 to 42 micrograms/ml) on the respective hepatic microsomal AHH activities.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008748	Methylcholanthrene	A carcinogen that is often used in experimental cancer studies.	20-Methylcholanthrene,3-Methylcholanthrene,20 Methylcholanthrene,3 Methylcholanthrene
D008806	Mice, Inbred AKR	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	Mice, AKR,Mouse, AKR,Mouse, Inbred AKR,AKR Mice,AKR Mice, Inbred,AKR Mouse,AKR Mouse, Inbred,Inbred AKR Mice,Inbred AKR Mouse
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008862	Microsomes, Liver	Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.	Liver Microsomes,Liver Microsome,Microsome, Liver
D011078	Polychlorinated Biphenyls	Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.	PCBs,Polychlorinated Biphenyl,Polychlorobiphenyl Compounds,Biphenyl, Polychlorinated,Biphenyls, Polychlorinated,Compounds, Polychlorobiphenyl
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004790	Enzyme Induction	An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.	Induction, Enzyme
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000072317	Polychlorinated Dibenzodioxins	Dibenzodioxin derivatives that contain multiple chloride atoms bound to the benzene ring structures.	TCDD,Tetrachlorodibenzodioxin,2,3,7,8-Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo-p-dioxins,Dibenzo(b,e)(1,4)dioxin, 2,3,7,8-tetrachloro-,PCDD,Polychlorinated Dibenzo-p-dioxins,Polychlorinated Dibenzodioxin,Polychlorodibenzo-4-dioxin,Polychlorodibenzo-p-dioxin,Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo p dioxins,Dibenzo-p-dioxins, Chlorinated,Dibenzo-p-dioxins, Polychlorinated,Dibenzodioxin, Polychlorinated,Dibenzodioxins, Polychlorinated,Polychlorinated Dibenzo p dioxins,Polychlorodibenzo 4 dioxin,Polychlorodibenzo p dioxin,Tetrachlorodibenzo p dioxin