-transparent.png){kind=logo}
This study demonstrates that the inhibition of adenylate cyclase activity by Ca2+ is enhanced in the presence of increasing [EGTA] (0, 0.3, 1, 2.5 mM) by 2 orders of magnitude. It has been established that this effect is not because of poor Ca2+ buffering by low [EGTA] or high Ca2+ binding by the membrane preparation. It is present irrespective of stimulus. We suggest the enhanced sensitivity of adenylate cyclase to Ca2+ induced by EGTA is caused by the Ca-EGTA complex being a more inhibitory species than Ca2+. Thus consideration of the effects of the Ca-EGTA complex should be made when interpreting the results from experiments involving Ca2+ and EGTA.
Related Publications
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
August 1985,
Archives of biochemistry and biophysics,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
March 1984,
Biochimica et biophysica acta,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
December 1986,
The Biochemical journal,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
January 1990,
Life sciences,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
July 1982,
The Biochemical journal,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
January 1986,
Neuroendocrinology,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
August 1978,
FEBS letters,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
October 1978,
Biochimica et biophysica acta,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
November 1978,
European journal of pharmacology,
J A Smith, and
M Griffin, and
S E Mireylees, and
R G Long
March 1981,
Clinical science (London, England : 1979),
