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The present study was undertaken to compare the technetium-99m labelled non-specific polyclonal human immunoglobulin (Ig) with 99mTc-labelled monomeric human immunoglobulin (m-Ig), 99mTc-labelled, protein A-purified, human immunoglobulin (A-Ig) and 99mTc-labelled monomeric, protein A-purified, human immunoglobulin (mA-Ig) as tracer agents for the detection of a thigh infection with Staphylococcus aureus. In vitro the binding of the various tracer agents to bacteria at various intervals was determined. For the in vivo evaluation, mice were infected and received one of the various labelled proteins. Scintigrams were made 0.25, 1, 4 and 24 h later. All 99mTc-labelled Igs bound to bacteria in vitro: the percentages of binding for the m-Ig (from 1 h onwards) and A-Ig and mA-Ig (from 3 h onwards) were significantly higher than that for Ig. The in vivo target-to-non-target (T/NT) ratios were significantly higher from 4 h onwards for all purified Igs than for Ig. Protein A-purified Igs yielded higher T/NT ratios than m-Ig. Furthermore, the amount of activity in the liver was significantly lower 24 h after administration of m-Ig, A-Ig and mA-Ig than after administration of Ig. It is concluded that in this experimental infection 99mTc-labelled monomeric Ig localizes a staphylococcal thigh infection better and faster than 99mTc-labelled unpurified Ig. However, the accumulation obtained with protein A-purified Ig or protein A-purified monomeric Ig was the highest of all tracer agents tested.
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
March 1991,
Journal of nuclear medicine : official publication, Society of Nuclear Medicine,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
January 1989,
Journal of immunological methods,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
August 1994,
The British journal of radiology,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
January 1984,
Folia microbiologica,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
January 1991,
Journal of nuclear biology and medicine (Turin, Italy : 1991),
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
December 1980,
Journal of immunology (Baltimore, Md. : 1950),
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
January 1989,
Experimental and clinical immunogenetics,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
January 1991,
European journal of nuclear medicine,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
December 1971,
The Journal of laboratory and clinical medicine,
W Calame, and
M Welling, and
H I Feitsma, and
G J Ensing, and
E K Pauwels
July 2012,
Clinical nuclear medicine,
