Biomaterial Database

Involvement of cellular adhesion sequences in the attachment of adenovirus to the HeLa cell surface. 1993

M T Belin, and P Boulanger

Laboratoire de Biochimie, Faculté de Médecine, Lille, France.

Immunofluorescence analysis of HeLa cells incubated with human adenovirus serotype 2 (Ad2) inoculum suggested that Ad2 receptors co-localized with the receptors of fibronectin (FNR) and vitronectin (VNR) at the cell surface. Ad2 adsorption also resulted in the occurrence of intracytoplasmic actin cables with submembranal anchorage. The cell binding of Ad2 virions, pentons and fibres was found to be efficiently inhibited by concanavalin A, laminin, anti-FNR and anti-VNR antibodies. Arginine-glycine-aspartyl tripeptide (RGD) and other related peptides reproducing cellular attachment sequences of adhesion proteins also competed with Ad2 for cell adsorption, and drastically reduced the virus progeny yield at the end of the infectious cycle. Data from binding competition assays with Ad2 virions showed that the apparent affinity constants of RGD motif-containing peptides for Ad2 receptor ranged from 0.75 x 10(8) M-1 to 2.2 x 10(8) M-1, with a number of peptide recognizing sites varying from 1.5 x 10(4) to 9 x 10(4) per cell for the different peptides studied. Polypeptide analysis of labelled plasma membrane fractions isolated after cross-linking to unlabelled Ad2 virions showed three major protein species with apparent M(r) of 130K, 60K and 44K, respectively, reacting with anti-FNR and anti-VNR antibodies. These results suggested that Ad2 and extracellular matrix proteins recognize similar adhesion sequences at the surface of HeLa cells, or alternatively that integrins and Ad2 receptors have overlapping ligand specificity.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009842	Oligopeptides	Peptides composed of between two and twelve amino acids.	Oligopeptide
D011991	Receptors, Virus	Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.	Viral Entry Receptor,Viral Entry Receptors,Virus Attachment Factor,Virus Attachment Factors,Virus Attachment Receptor,Virus Attachment Receptors,Virus Entry Receptor,Virus Entry Receptors,Virus Receptor,Virus Receptors,Attachment Factor, Virus,Attachment Factors, Virus,Attachment Receptor, Virus,Attachment Receptors, Virus,Entry Receptor, Viral,Entry Receptor, Virus,Entry Receptors, Viral,Entry Receptors, Virus,Receptor, Viral Entry,Receptor, Virus,Receptor, Virus Attachment,Receptor, Virus Entry,Receptors, Viral Entry,Receptors, Virus Attachment,Receptors, Virus Entry
D002448	Cell Adhesion	Adherence of cells to surfaces or to other cells.	Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000260	Adenoviruses, Human	Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G.	APC Viruses,APC Virus,Adenovirus, Human,Human Adenovirus,Human Adenoviruses
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).