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To analyze phagocytosis mechanism of granulocyte using fluorescent microspheres, changes of phagocytic index, complement receptors, Fc receptors and adhesion molecules on cell membrane, after exogenous stimulation were determined by flow cytometry. The changes of phagocytic index by blocking test was also determined using monoclonal antibodies for receptors. The phagocytic index was decreased by supernatant of S. aureus, and was increased by supernatant of E. coli and phorbol myristate acetate (PMA). By PMA stimulation, surface marker positive cells increased in CR1, IgGFcR2 and decreased in IgGFcR3, LFA-3, Homing receptor, whereas they increased in CR1, IgMFcR, LFA-1 beta and decreased in LFA-3 by the supernatants of S. aureus and E. coli. Blocking test using monoclonal antibodies to individual receptors showed decrease of phagocytic index in CR1, CR3, LFA-1 beta. In this phagocytosis using fluorescent microspheres, the complement receptors were most dedicating in phagocytosis of granulocyte.
H Sakai, and
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Y Katayama, and
T Matsuyama
February 1983,
Blood,
H Sakai, and
K Mori, and
Y Katayama, and
T Matsuyama
January 1986,
Zeitschrift fur arztliche Fortbildung,
H Sakai, and
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January 1985,
Transfusion,
H Sakai, and
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Y Katayama, and
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December 1977,
Blood,
H Sakai, and
K Mori, and
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January 1985,
Dermatologische Monatschrift,
H Sakai, and
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May 1990,
Arzneimittel-Forschung,
H Sakai, and
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January 1974,
Verhandlungen der Deutschen Gesellschaft fur Innere Medizin,
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January 1980,
Scandinavian journal of infectious diseases,
H Sakai, and
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June 1949,
Journal of bacteriology,
H Sakai, and
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January 2012,
Hepato-gastroenterology,
