Biomaterial Database

New observations on proteases of the human hookworm Necator americanus. 1993

S Kumar, and D I Pritchard

Department of Life Science, University of Nottingham, University Park.

Two protease populations have been identified in the somatic products of adult Necator americanus. One population (158, 138, 34 and 31 kDa) loses, whereas the other population (107, 74, 51 and 20 kDa) retains, its proteolytic activity after elution from substrate gels. The present study warns that hookworm proteases may oligomerise and/or lose their activity under the conditions necessary for their purification. We suggest that following assessment of protease activity on substrate gels, the protease of interest should be eluted from plain gels; their isolation from substrate gels may lead to a reduced or no yield.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010450	Endopeptidases	A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.	Endopeptidase,Peptide Peptidohydrolases
D011108	Polymers	Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).	Polymer
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017221	Necator americanus	A common parasite of humans in the moist tropics and subtropics. These organisms attach to villi in the small intestine and suck blood causing diarrhea, anorexia, and anemia.	Hookworm, New World,Hookworms, New World,New World Hookworm,New World Hookworms