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A refined method for the measurement of protein-bound iodine (PBI) in blood serum was demonstrated by the use of epithermal neutron activation analysis (ENAA). PBI in ammonium sulfate-precipitated serum protein, after epithermal neutron activation, was determined by high resolution gamma-ray spectrometry. From our results, the PBI concentration was 67.8 +/- 2.2 ng/mL. Good agreement was obtained with published data, ranging from 40 to 80 ng/mL, which had been obtained using different analytical techniques. The validity of these techniques for PBI has been born out by a very good accuracy and simplicity without temperature dependence.
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