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Two DNA repair enzymes for 8-hydroxyguanine (also known as 7,8-dihydro-8-oxoguanine; OH8Gua, oxo8Gua) have been identified in human HeLa cell nuclear extract. One is OH8Gua-glycosylase and the other is OH8Gua-endonuclease that lacks OH8Gua-glycosylase activity. They were separated by heparin-Sepharose column chromatography and characterized by endonuclease nicking assay or by measuring the OH8Gua released from substrate DNA using high pressure liquid chromatography-electrochemical detection. Both OH8Gua repair enzymes act only on the OH8Gua-containing strand of the duplex substrate DNA containing OH8Gua/C, OH8Gua/T, or OH8Gua/G. DNA containing OH8Gua/A base pair was very poor substrate for either enzymes. OH8Gua-endonuclease simultaneously cleaves phosphodiester bonds on both sides of the OH8Gua residue, leaving 5'-hydroxy and 3'-hydroxy groups.
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
January 1992,
Nucleic acids symposium series,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
February 2000,
The Journal of biological chemistry,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
October 1997,
Mutation research,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
January 2001,
Mutation research,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
January 2000,
Nucleic acids symposium series,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
October 1992,
Journal of bacteriology,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
May 2002,
Nucleic acids research,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
November 1992,
Nucleic acids research,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
January 1995,
Proceedings of the National Academy of Sciences of the United States of America,
T Bessho, and
K Tano, and
H Kasai, and
E Ohtsuka, and
S Nishimura
February 1996,
Biochemistry,
