-transparent.png){kind=logo}
Quantification of HIV-1 viral RNA in plasma was achieved by competitive co-amplification of a dilution series of in vitro generated RNA using the nucleic acid sequence based amplification (NASBA) technology. This 1.5 kilobase in vitro RNA, comprising the gag and part of the pol region, differs only by sequence-randomization of a 20 nt fragment from the wild-type RNA, ensuring equal efficiency of amplification. In model systems the accuracy of this method is within one log. Application of the Q-NASBA to plasma samples of a patient with a primary HIV-1 infection shows good concordance of the HIV-1 RNA profile with the p24 antigen profile. However, the HIV-1 RNA determination is more sensitive than the p24 antigen determination. Peak values of HIV-1 RNA are around 10(8) RNA molecules per ml plasma at the moment of seroconversion. Quantitative nucleic acid detection methods, like Q-NASBA, allow the monitoring of HIV-1 RNA during the course of infection which might have predictive value for disease development.
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
October 1996,
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
January 1998,
Journal of medical virology,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
December 1996,
AIDS (London, England),
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
March 1995,
Journal of virological methods,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
August 1995,
Journal of virological methods,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
May 1996,
Journal of clinical microbiology,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
February 1999,
AIDS (London, England),
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
January 2011,
Antiviral therapy,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
September 1997,
Revista espanola de fisiologia,
B van Gemen, and
T Kievits, and
R Schukkink, and
D van Strijp, and
L T Malek, and
R Sooknanan, and
H G Huisman, and
P Lens
May 2003,
Journal of acquired immune deficiency syndromes (1999),
