Biomaterial Database

Synthesis of free X duplications carrying a specific region of the centromeric heterochromatin in Drosophila melanogaster. 1993

H S Park, and M T Yamamoto

Division of Biology, Miyazaki Medical College, Japan.

Free X duplication chromosomes of Drosophila melanogaster were synthesized by X-ray irradiating the In(1)scL8Lsc8R chromosome which has a deletion in the distal half of hA and the proximal half of hB of the centromeric heterochromatin. Fifty-nine duplications have been isolated and cytogenetically analyzed. They all carry wild-type allele of the yellow gene, y+, which should come from the distal tip of In(1)scL8Lsc8R. They appear to be telocentric and predominantly heterochromatic. Majority of the duplications, especially in the classes MEDIUM and LARGE, can pair with XYL.YS in the male meiosis, indicating that they carry male meiotic pairing site(s) that is known to be located exclusively in the X heterochromatin. Complementation test in the males, Df(1)svr, v/Dp, y+, demonstrates that most of the duplications in the classes MEDIUM and LARGE carry euchromatin enough to cover the deletion. The portion of the euchromatin should be of the very proximal region close to the irradiated X chromosome centromere.

UI	MeSH Term	Description	Entries
D007621	Karyotyping	Mapping of the KARYOTYPE of a cell.	Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D008297	Male		Males
D008540	Meiosis	A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.	M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D002503	Centromere	The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.	Centromeres
D002875	Chromosomes	In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Chromosome
D004331	Drosophila melanogaster	A species of fruit fly frequently used in genetics because of the large size of its chromosomes.	D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D005260	Female		Females
D005810	Multigene Family	A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)	Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D006570	Heterochromatin	The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE.	Heterochromatins
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia