-transparent.png){kind=logo}
The RIB7 gene encoding the enzyme of the final stage of riboflavin biosynthesis in Pichia guilliermondii--riboflavin synthase was cloned on the pFL38 shuttle vector as the Sau3A fragment of the chromosomal DNA of about 4 kb. The HindIII fragment of 1.4 kb was subcloned from the hybrid plasmid pFR7 obtained onto the pUC18 plasmid. The plasmid pR7 thus constructed transform Escherichia coli ribB-45 mutant cells with a blocked riboflavin synthase approximately at the same frequency as pFR7. High riboflavin synthase activity was discovered in the E. coli transformants carrying pR7 but not pFR7. Using both plasmids we also complemented rib17 mutant of P. guilliermondii.
Related Publications
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
January 2002,
TSitologiia i genetika,
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
January 1997,
Ukrainskii biokhimicheskii zhurnal (1978),
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
January 1980,
Zeitschrift fur allgemeine Mikrobiologie,
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
July 2014,
Current microbiology,
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
February 1976,
Biokhimiia (Moscow, Russia),
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
December 1978,
Biokhimiia (Moscow, Russia),
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
August 1995,
Yeast (Chichester, England),
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
October 1981,
Biokhimiia (Moscow, Russia),
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
January 2000,
Ukrains'kyi biokhimichnyi zhurnal (1999 ),
E M Logvinenko, and
Iu Z Stasiv, and
M L Zlochevskiĭ, and
A Ia Voronovskiĭ, and
M Iu Beburov, and
G M Shavlovskiĭ
January 2002,
Prikladnaia biokhimiia i mikrobiologiia,
