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We studied whether a novel vasoconstrictor, endothelin-1 (ET-1), is synthesized by and released from porcine granulosa cells, and whether ET-1 acts directly on granulosa cells in an autocrine/paracrine fashion. The dilution curve of the conditioned medium from cultured porcine granulosa cells was parallel to a standard curve of ET-1 in RIA. Reverse-phase HPLC of the conditioned media from the granulosa cells revealed a major peak of ET-1-like immunoreactivity (ET-1-LI) coeluting with standard ET-1. ET-1-LI was released from cultured porcine granulosa cells as a function of time. Northern blot analysis demonstrated the expression of mRNA for prepro-ET-1 in the granulosa cells. We demonstrated the presence of ET-1 in the follicular fluid of porcine ovaries, and that the concentration of ET-1 in large follicles was higher than that in small-medium follicles. ET-1 dose-dependently stimulated cell growth and DNA synthesis in porcine granulosa cells. ET-1 inhibited the FSH- and hCG stimulated accumulation of progesterone in porcine granulosa cells in long-term incubation. These findings suggest that ET-1 produced by porcine granulosa cells may function as an autocrine/paracrine growth factor and modulator of steroidogenesis in ovarian granulosa cells.
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
January 2000,
Annals of the New York Academy of Sciences,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
December 1992,
The Journal of clinical endocrinology and metabolism,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
November 1997,
Hypertension (Dallas, Tex. : 1979),
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
October 2003,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
December 1998,
Endocrinology,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
May 1991,
The Journal of clinical investigation,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
January 2002,
The Journal of clinical endocrinology and metabolism,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
May 2011,
Biology of reproduction,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
January 1991,
Journal of cardiovascular pharmacology,
S Kamada, and
T Kubota, and
Y Hirata, and
T Imai, and
K Ohta, and
M Taguchi, and
F Marumo, and
T Aso
January 1993,
Reproduction, fertility, and development,
