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Sheep beta-lipotropin (beta-LPH) (sequence 1-91) was selectively cleaved with trypsin after blocking the epsilon-amino groups of lysine with citraconic anhydride. The resulting peptides were purified by a combination of cation-exchange chromatography and high-voltage electrophoresis. The purified fragments were then tested for their morphine-like activity in the mouse vas deferens bioassay. The active peptides were 61-91 and 61-80 were about as active as the synthetic methionine-enkephalin, and in turn these were about 100 times more active than beta-LPH itself. The inhibition of electrically stimulated mouse vas deferens by these peptides is reversed by naloxone, and suggests a competitive character of interaction. It is thus concluded that the active core for the morphine like activity in the mouse vas deferens bioassay is the fragment 61-65 of beta-LPH.
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
April 1976,
FEBS letters,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
September 1976,
Proceedings of the National Academy of Sciences of the United States of America,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
September 1976,
Life sciences,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
June 1976,
Proceedings of the National Academy of Sciences of the United States of America,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
January 1976,
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
July 1981,
The Journal of biological chemistry,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
March 1980,
The Journal of clinical endocrinology and metabolism,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
July 1981,
Pharmacology, biochemistry, and behavior,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
April 1986,
Archives of biochemistry and biophysics,
N G Seidah, and
M Lis, and
C Gianoulakis, and
R Routhier, and
S Benjannet, and
P W Schiller, and
M Chretien
February 1991,
Biophysical chemistry,
